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目的探索连续多次镉染毒后雄性小鼠的生殖毒性。方法将雄性NIH小鼠随机分为正常对照组、低、中、高剂量组共4组,每组6只动物,分别按0、0.17、0.34、0.68 mg Cd/kg的剂量腹腔注射氯化镉溶液,1次/d,连续5 d。染毒结束后观察120 d。雄鼠与雌鼠合笼测定交配能力,进行精子计数并观察精子畸形率;测定血清中睾酮、睾丸中镉含量;观察睾丸及附睾的病理变化。结果与正常对照组相比,中、高剂量组雄鼠附睾精子浓度和血清中睾酮水平均显著降低(P<0.05);低、中、高3个剂量组附睾中精子畸形率及睾丸中镉含量均显著升高(P<0.05);3个剂量组雄鼠的交配能力差异无统计学意义(P>0.05);部分动物的睾丸组织镜下发现边缘生精小管萎缩;各组动物的附睾中均未发现明显异常病理改变。结论雄性小鼠脱离接触镉后4个月仍可见生殖毒性。
Objective To explore the reproductive toxicity of male mice after repeated cadmium exposure. Methods Male NIH mice were randomly divided into normal control group, low, medium and high dose group of 4 groups, each group of 6 animals, respectively, 0,0.17,0.34,0.68 mg Cd / kg dose of intraperitoneal injection of cadmium chloride Solution, 1 time / d, continuous 5 d. After the end of the observation of 120 d. Male and female mice were caged to determine mating ability, sperm counts and sperm deformity were observed. The contents of cadmium in serum and testis were measured. Pathological changes of testis and epididymis were observed. Results Compared with the normal control group, the sperm concentration of epididymis and the level of serum testosterone were significantly decreased in the middle and high dose groups (P <0.05). In the low, middle and high dose groups, the sperm deformity rate in the epididymis and cadmium (P <0.05). There was no significant difference in copulation ability between male and female rats at 3 doses (P> 0.05). Some seminiferous tubules of atrophy of marginal seminiferous tubules were observed under testicular microscopy. No significant abnormal pathological changes were found. Conclusion Male reproductive toxicity can still be seen at 4 months after exposure to cadmium.