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将1979年正式定名建株到现在,其间经历了9年反复冻存、复苏、培养的FCC-1/HN株恶性疟原虫,用培养效果明显优于成人血清的10%脐带血清进行体外培养。60天后取培养物用透射电镜观察了疟原虫和其宿主细胞——红细胞近期的超微结构情况。观察结果表明与以往已报道的同株疟原虫相比,除见到了仍有相似之处外;还发现有某些不同的特征性改变:①疟原虫寄生的红细胞,其表面结节完全消失;也未见到典型的茂氏裂隙。②疟原虫虫体胞质内空泡结构和胞质裂隙增多。提示:疟原虫和被寄生的红细胞的超微结构在外界环境条件改变时,也会随之发生一些变化。这些变化,应引起我们今后在以体外培养研究疟原虫的生物学、生理生化和药物筛选中的重视,尤其是免疫学的重视。
The plant was officially established in 1979 up to now, during which it has undergone 9 years of repeated cryopreservation, resuscitation and training of the FCC-1 / HN strain of Plasmodium falciparum, and cultured in vitro with 10% cord serum whose culturing effect is obviously better than that of adult serum. After 60 days, the culture was observed by transmission electron microscopy of Plasmodium and its host cell - the recent ultrastructure of red blood cells. Observations show that compared with the same strain of Plasmodium that has been reported in the past, there are some similarities in addition to those seen in the past: (1) Plasmodium parasitic erythrocytes completely disappear from the surface nodules; Also did not see the typical Mau fracture. ② Plasmodium parasitic cytoplasmic vacuoles and cytoplasmic cracks increased. Tip: Plasmodium parasites and the ultrastructure of red blood cells in the external environmental conditions change, there will be some changes. These changes should cause us to pay more attention to biology, physiology, biochemistry and drug screening of in vitro culture research of Plasmodium, especially immunology.