目的 观察苯乙酸诱导分化胶质瘤细胞C6过程中，对细胞形态的影响。方法 采用醋酸双氧铀电子染色，电子显微镜(电镜)下观察培养C6细胞在应用苯乙酸后的超微结构变化。结果 应用苯乙酸后，C6细胞核形变规整，由畸形核变为类圆形。胞浆中代表细胞分化程度的粗面内质网大量增生。结论 苯乙酸可在亚细胞水平使C6细胞分化程度提高，恶性度降低。 Objective To observe the effect of phenylacetate on the differentiation of glioma cells C6. Methods Electron staining with uranyl acetate and electron microscopy (SEM) were used to observe the ultrastructural changes of cultured C6 cells treated with phenylacetic acid. Results After the application of phenylacetic acid, the nuclear deformation of C6 cells was regular and changed from a deformed nucleus to a round shape. The rough endoplasmic reticulum, which represents the degree of cell differentiation in the cytoplasm, proliferated. Conclusion Phenylacetic acid can increase the differentiation of C6 cells at the subcellular level and reduce the degree of malignancy.