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用毛细管电泳以聚环氧乙烷(PEO)为筛分介质对pUC19 DNA/Msp Ⅰ(HpaⅡ)Marker中的12条DNA片段进行了分离,并尝试用Ogston模型、爬行模型以及线性模型对分离机理进行研究,最终发现26～147 bp的小片段,在低电场强度时能很好地符合Ogston模型理论,而190～501 bp中等长度的DNA片段电泳迁移率与其尺寸间存在很好的负相关的线性关系,为此,提出一种新的线性模型来进行解释.此外,还探讨了PEO的浓度和电场强度对分离的影响.其结论可更好地从理论上指导对中小片段DNA的分离,对肿瘤基因突变点的分析和PCR扩增产物的分离分析具有重要的意义.“,”The 12 segments of pUC19 DNA/MspⅠ (HpaⅡ) marker have been separated and theoretical ex-planation was provided. The results gave better insight into the mechanisms of separations in polymer solu-t-ions. The dependence of different parameters on DNA size, electric field and the polymer concentration was evaluated and explained. Ogston model and a linear model were used to explain the behavior of 26～147 bp DNA fragmtent at lower electric field intensity. But, a new linear model was established in our experiment, which included the negative linear relation of electrophoretic mobility with DNA size. It could explain the separation mechanisms of 190～501 bp DNA fragment. The data should help to optimize and direct the small and medium DNA molecule separation in capillary electrophoresis and have a great sense in analysis of the mutational site of the tumor and the product of PCR.