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从一例患低分化的原始淋巴细胞性淋巴瘤患者的骨髓,分离培养出具有特殊性质的新细胞系(K-LL-3)并在软琼脂培养基上传代一年余。K-LL-3细胞系与患者原发肿瘤在形态学、细胞化学染色方面极其相似。但该细胞的生长性质、染色体、细胞表面受体和EB病毒的研究与非恶性类淋巴母细胞系有明显区别:K-LL-3细胞EB病毒阴性,有伪二倍体46XY核型、不能分泌免疫球蛋白,不具备表面免疫球蛋白。而非恶性类淋巴母细胞系(LCL)具有二倍体的核型、EB病毒阳性、有表面免疫球蛋
From a patient with poorly differentiated primary lymphoid lymphoma, a new cell line (K-LL-3) was isolated and cultured for more than one year on soft agar medium. The K-LL-3 cell line is very similar to the patient’s primary tumor in morphology and cytochemical staining. But the cell growth properties, chromosomes, cell surface receptors, and Epstein-Barr virus were significantly different from non-malignant lymphoblastoid cell lines: EBV-negative K-LL-3 cells had a pseudo-diploid 46XY karyotype, Secretion of immunoglobulins, do not have surface immunoglobulin. Non-malignant lymphoblastoid cell lines (LCL) have diploid karyotypes, Epstein-Barr virus positive, surface immunoglobulin