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目的建立LC-MS/MS法测定犬血浆中长春瑞滨的浓度并进行药动学研究。方法将犬血浆经甲醇蛋白沉淀处理后稀释进样。色谱柱为Agilent SB-C_(18)柱;流动相为甲醇-2 mmol·L~(-1)醋酸铵水溶液-甲酸(75:25:0.1);流速为0.3 mL·min~(-1)。采用电喷雾离子源,正离子方式多反应监测(MRM)扫描分析。检测离子通道分别为m/z 779.5→658.5(长春瑞滨)和m/z 309.1→163.2(华法林)。结果长春瑞滨的线性范围为1~100μg·L~(-1)(R~2>0.99),定量限为1μg·L~(-1)。批内和批间精密度均<5.87%,高、中、低浓度的方法回收率为96.55%~102.2%。结论本方法操作简捷,灵敏度高,专属性好,适用于长春瑞滨的犬体内药动学研究。
Objective To establish a LC-MS / MS method for the determination of vinorelbine in canine plasma and study its pharmacokinetics. Methods The canine plasma was diluted with methanol and then precipitated. The column was Agilent SB-C_ (18) column. The mobile phase was methanol-2 mmol·L -1 ammonium acetate solution-formic acid (75:25: 0.1), the flow rate was 0.3 mL · min -1 . Electrospray ionization, positive ion multi-reaction monitoring (MRM) scanning analysis was used. The ion channels tested were m / z 779.5 → 658.5 (vinorelbine) and m / z 309.1 → 163.2 (warfarin), respectively. Results The linear range of vinorelbine was 1 ~ 100 μg · L ~ (-1) (R ~ 2> 0.99), and the limit of quantification was 1 μg · L ~ (-1). The intra- and inter-batch precision were <5.87%. The recoveries of high, medium and low concentrations were 96.55% ~ 102.2%. Conclusion The method is simple, sensitive and specific, and is suitable for vinorelbine pharmacokinetics studies in dogs.