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目的:探讨caspase 8在热疗降低胶质瘤侵袭性过程中的作用。方法:利用Transwell构建胶质瘤侵袭模型,随机分为热疗对照组以及热疗30、60、120、180 min和240 min组。采用流式细胞术检测胶质瘤细胞凋亡水平,免疫印迹法检测胶质瘤细胞caspase 8的表达水平。结晶紫染色法测定胶质瘤侵袭性变化。结果:(1)热疗各组胶质瘤细胞凋亡率均明显高于热疗对照组(P<0.05);(2)各热疗组胶质瘤细胞的caspase 8表达均高于热疗对照组,有统计学差异(P<0.05);(3)各热疗组胶质瘤细胞的侵袭性均较对照组降低(P<0.05)。结论:热疗后胶质瘤细胞侵袭性降低,这可能与caspase 8表达增加导致胶质瘤细胞凋亡有关。
Objective: To investigate the role of caspase 8 in hyperthermia in glioma invasiveness. Methods: Glioma invasion model was established by Transwell. The rats were randomly divided into hyperthermia control group and hyperthermia groups of 30, 60, 120, 180 min and 240 min. The apoptosis of glioma cells was detected by flow cytometry. The expression of caspase 8 in glioma cells was detected by Western blotting. Crystalline Violet Staining Assay for Invasive Glioma Invasion. Results: (1) The apoptosis rates of glioma cells in hyperthermia groups were significantly higher than those in hyperthermia control groups (P <0.05). (2) The expression of caspase 8 in glioma cells was higher than that in hyperthermia group (P <0.05). (3) The invasion of glioma cells in each hyperthermia group was lower than that in control group (P <0.05). Conclusion: The invasiveness of glioma cells decreased after hyperthermia, which may be related to the increased expression of caspase 8 leading to glioma cell apoptosis.