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为了探索粗枝云杉胚性组织对表油菜素内酯的生理生化响应,本研究以粗枝云杉3个基因型未成熟胚为供试材料,通过在诱导培养基中添加不同浓度EBR(0,0.5 μmol/L,1.0 μmol/L),经诱导后获得胚性组织,然后进行增殖培养,在同一增殖周期的第0d和第10d时,比较胚性组织中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性以及丙二醛(MDA)和可溶性蛋白含量的变化,结果表明,实验组与对照组相比,在第0天时,胚性组织中POD活性、CAT活性、MDA含量以及可溶性蛋白含量均升高了,而SOD活性降低了;在第10天时,胚性组织中SOD活性、CAT活性、MDA含量以及可溶性蛋白含量均升高了,而POD活性降低了.研究结果为探讨粗枝云杉胚性组织的生理生化变化建立基础资料,也为提高粗枝云杉胚性组织诱导率提供参考依据.“,”The study investigated the interaction relationship between different genotypes embryonic tissues of Picea asperata Mast.under epibrassinolide (EBR) induction.Embryonic tissues were induced from immature zygotic embryos of three genotypes ofP.asperata by adding different concentrations EBR (0,0.5 μmol/L,1.0 μmol/L) in the induction medium.In the same proliferation cycle at the 0 and 10 d,the different activities of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT),as well as the contents of malondialdehyde (MDA) and soluble protein were examined.The results showed that compared with the control group,on the 0th d the POD,CAT activity and MDA,soluble protein content of experimental group were higher,but the activity of SOD was lower;on the 10th d the SOD,CAT activity and MDA,soluble protein content of experimental group were higher,but the activitie of POD was lower.The results would establish a basic data for the biochemical and physiological changes study of embryonic tissues,and provide evidence for the improvement of embryonic tissue induction rate ofP.asperata.