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在 Wistar 大鼠乳鼠心室肌单个细胞上,用连细胞电压钳法,观察人参三醇组皂甙单体 Rg_2 60μmol/L 对钙通道单通道活动的影响,并与钙通道阻滞剂异博定79μmol/L、钙通道激动剂 Bay K 8644 5μmol/L 对照,证明 Rg_2对钙通道有阻滞作用,其机制在于使钙通道的开放时间缩短、开放概率减少、关闭时间延长,而对 Ba~(2+)流幅值无明显影响。用电子自旋共振法(ESR)测定了培养心肌细胞的自由基含量,Rg_230μmol/L 能显著抑制黄嘌吟0.42nmol/L、黄嘌呤氧化酶5.3nmol/L 所致的自由基含量的增多。
The effects of ginsenoside Rg_2 60μmol/L on the single channel activity of calcium channels were observed on single cells of Wistar rats’ ventricular myocytes with voltage-clamp method, and the results were compared with that of calcium channel blockers. 79μmol/L, calcium channel agonist Bay K 8644 5μmol/L control, proved that Rg_2 has a blocking effect on calcium channels. The mechanism is that the calcium channel opening time is shortened, the opening probability is reduced, and the closing time is prolonged. The 2+) flow amplitude has no significant effect. The free radical content of cultured cardiomyocytes was determined by electron spin resonance (ESR). Rg_230μmol/L could significantly inhibit the increase of free radical content caused by astragalus 0.42nmol/L and xanthine oxidase 5.3nmol/L.