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以聚丙烯酰胺凝胶电泳方法研究了荞麦属植物8个种42个收集系干种子和发芽种子的淀粉酶和甲酸脱氢酶同功酶。结果表明,荞麦淀粉酶在于种子中缺乏活性,但是在发芽种子中活性很强。在供试材料的发芽种子中共发现23个淀粉酶谱带,其中甜荞和苦荞分别有10条和8条。不同荞麦种间淀粉酶谱带差异很大,但是同种内不同收集系间差异较小。谱带聚类分析表明大野荞和毛野荞分别与甜荞和苦荞较近缘,支持它们分别为甜荞和苦荞祖先种的假说。在干种子和发芽种子中,发现所有荞麦种类均只有1条位置一致的甲酸脱氢酶谱带,暗示该酶在进化中具有高度稳定性。
The amylase and formate dehydrogenase isoenzyme of dry seeds and germinating seeds collected from 8 species of 8 buckwheat plants were studied by polyacrylamide gel electrophoresis. The results show that buckwheat amylase is inactive in seeds but highly active in germinating seeds. A total of 23 amylase bands were found in the germinating seeds of the tested materials, of which 10 were for buckwheat and 8 for buckwheat respectively. There were significant differences in amylase bands among different buckwheat species, but there was little difference among the different collection lines within the same species. Cluster analysis showed that the wild buckwheat and hairy buckwheat buckwheat and buckwheat, respectively, are more closely related to support them respectively buckwheat and buckwheat ancestral hypothesis. In dry seeds and germinating seeds, only one locus of formate dehydrogenase was found in all buckwheat species, suggesting that the enzyme is highly stable in evolution.