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目的:为了解P53抑癌基因在溃疡性结肠炎、结肠直肠癌及癌旁组织中的改变状况,进而探讨其中癌变过程中所起的作用。方法:采用改进的非同位素PCR-SSCP方法对21例溃疡性结肠炎、19例结肠直肠癌(CRC)及癌旁组织P53基因第5、6外显子(Exon 5.6)进行检测,并应用DNA测序技术对PCR-SSCP阳性标本进行进一步测定。结果:21例溃疡性结肠炎组织中有6例检出P53基因改变,19例CRC组织中有7例检出P53基因点突变,2例DNA缺失;19例CRC癌旁组织6例检出P53基因P53点突出未检出DNA缺失。结论:P53基因改变在CRC及癌旁组织存在较为普遍,而在溃疡性结肠炎也有一定的检出率,表明P53基因改变在溃疡性结肠炎相关性结肠直肠癌(UCACRC)是早期事件,并可能是UCACRC的发病原因之一。
OBJECTIVE: To understand the role of P53 tumor suppressor gene in ulcerative colitis, colorectal cancer, and adjacent tissues, and to explore the role of P53 tumor suppressor gene in carcinogenesis. METHODS: A modified non-isotopic PCR-SSCP method was used to detect 21 cases of ulcerative colitis, 19 cases of colorectal cancer (CRC), and P53 exon 5 and exon 6 (Exon 5.6). Sequencing technology was used to further determine PCR-SSCP positive samples. RESULTS: P53 gene mutation was detected in 6 of 21 cases of ulcerative colitis. P53 gene mutations were detected in 7 of the 19 CRC tissues and DNA was absent in 2 cases. P53 was detected in 19 cases of CRC adjacent tissues in 19 cases. The P53 spot highlights no DNA deletions. CONCLUSIONS: P53 gene alterations are more common in CRC and paracancerous tissues, and there is also a certain detection rate in ulcerative colitis, suggesting that P53 gene alterations are an early event in ulcerative colitis-associated colorectal cancer (UCACRC) and It may be one of the reasons for the onset of UCACRC.