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目的:用HPLC法测定不同大小丹蓝微丸中丹皮酚的含量。方法:色谱柱为Agilent TC-C18(250mm×4.6mm,5μm),流动相为甲醇-水(65∶35),流速为1.000mL·min-1,检测波长为274nm。结果:丹皮酚在0.8128~4.064μg范围内与峰面积积分值呈良好线性关系(R2=0.9998);加样回收率为97.24%(RSD=1.95%)。结论:该法简便、准确、重复性好,可用于丹蓝微丸中丹皮酚的含量测定,随着微丸直径的减小,丹皮酚的含量有一定增加。
Objective: To determine the content of paeonol in different size dan blue pellets by HPLC method. Methods: The column was Agilent TC-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (65:35) and the flow rate was 1.000 mL · min-1. The detection wavelength was 274 nm. Results: Paeonol had a good linear relationship with the peak area integral in the range of 0.8128 ~ 4.064μg (R2 = 0.9998). The recovery rate was 97.24% (RSD = 1.95%). Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of paeonol in Danlan pellets. As the diameter of the pellets decreases, the content of paeonol increases.