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目的探讨肝细胞癌（ＨＣＣ）及其癌旁肝组织中丙型肝炎病毒（ＨＣＶ）ＮＳ３蛋白对Ｐ５３蛋白表达的影响及其在肝癌发生中的作用。方法用免疫组织化学技术检测４７例乙型肝炎病毒阴性的ＨＣＣ及其癌旁肝组织中ＨＣＶＮＳ３蛋白和Ｐ５３蛋白的表达。结果ＨＣＶＮＳ３蛋白在ＨＣＣ中的阳性率（６２％）明显低于癌旁肝组织（８３％），Ｐ＜００２５。癌组织中其表达强度与癌细胞分化程度呈相关性（Ｐ＜００２５）。Ｐ５３蛋白在癌组织中的阳性率（８１％）明显高于癌旁组织（４７％），Ｐ＜００２５；癌细胞分化愈差，表达愈强（Ｐ＜００５）；癌组织中Ｐ５３蛋白表达与ＨＣＶＮＳ３蛋白的表达无相关性（Ｐ＞０５），而癌旁肝组织中两者表达呈显著相关性（Ｐ＜００１），ＨＣＶＮＳ３蛋白阳性患者中Ｐ５３蛋白表达明显高于ＨＣＶＮＳ３阴性病例，Ｐ＜００５。结论ＨＣＶＮＳ３蛋白可能是在肝细胞转化早期通过内源性机制间接作用于Ｐ５３基因使其突变导致肝细胞癌变。
Objective To investigate the effect of hepatitis C virus (HCV) NS3 protein on the expression of P53 protein in hepatocellular carcinoma (HCC) and its surrounding liver tissue and its role in the development of hepatocellular carcinoma. Methods Immunohistochemical technique was used to detect the expression of HCVNS3 protein and P53 protein in 47 cases of hepatitis B virus-negative HCC and its adjacent liver tissues. Results The positive rate of HCV NS3 protein in HCC (62%) was significantly lower than that in para-carcinoma tissue (83%), P<0025. The intensity of expression in cancer tissues was correlated with the degree of cancer cell differentiation (P<0025). The positive rate of P53 protein in cancer tissues (81%) was significantly higher than that in adjacent tissues (47%), P<0025; the worse the differentiation of cancer cells, the stronger the expression (P<005); There was no correlation between P53 protein expression and HCV NS3 protein expression (P>0.05), but there was a significant correlation between the expression of the two proteins in the adjacent liver tissue (P<0.01). The expression of P53 protein was significantly higher in HCV NS3 protein positive patients. In HCV NS3 negative cases, P<005. Conclusion The HCVNS3 protein may be indirectly affected by the P53 gene in the early stage of hepatocyte transformation through an endogenous mechanism, resulting in mutation of hepatocyte.