The present study conducted a multi-scale dynamic functional connectivity analysis to evaluate dynamic behavior of acupuncture at Taichong (LR3) and sham acupoints surrounding Taichong. Results showed differences in wavelet transform coherence characteristic curves in the declive, precuneus, postcentral gyrus, supramarginal gyrus, and occipital lobe between acupuncture at Taichong and acupuncture at sham acupoints. The differences in characteristic curves revealed that the specific effect of acupuncture existed during the post-acupuncture rest state and lasted for 5 minutes.