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利用图像分析技术，对１５例正常肠粘膜、４５例癌旁粘膜、３９例大肠腺瘤及６６例大肠癌进行了Ｐ５３基因产物免疫组化染色结果及七项核参数的测定，并对二者进行线性相关分析。结果表明：正常粘膜、癌旁粘膜、大肠腺瘤及大肠癌Ｐ５３基因产物表达阳性率及免疫反应分值（ｉｍｍｕｎｏｒｅａｃｔｉｖｅｓｃｏｒｅＩＲＳ）分别为：０、４４４％、１０２６％、５７５８％；０、０３５、０７６、１０２６。相关分析表明：Ｐ５３免疫组化染色强度及阳性物含量与其相应的细胞核大小及ＤＮＡ含量呈正相关。该结果提示：Ｐ５３基因突变可能与大肠癌的发生有关。Ｐ５３免疫组化染色结合图像分析技术进行ＤＮＡ含量测定，对大肠癌的早期诊断有重要意义。
Using image analysis techniques, immunohistochemical staining of P53 gene products and determination of seven nuclear parameters were performed on 15 normal intestinal mucosa, 45 paraneoplastic mucosa, 39 colorectal adenomas, and 66 colorectal cancers. Perform linear correlation analysis. The results showed that the positive rate of P53 gene product expression and immunoreactivescore (IRS) in normal mucosa, paraneoplastic mucosa, colorectal adenoma and colorectal cancer were: 0, 4 44%, 10 26%, 57 58%; 0, 035, 076, 1026. The correlation analysis showed that the intensity of P53 immunohistochemical staining and the content of positive substances were positively correlated with their corresponding nuclei size and DNA content. The results suggest that P53 gene mutation may be related to the occurrence of colorectal cancer. P53 immunohistochemical staining combined with image analysis techniques for DNA content determination is important for the early diagnosis of colorectal cancer.