论文部分内容阅读
目的分析上饶市2013-2014年甲型H1N1流感病毒M2基因以及NA基因的特点,掌握其耐药情况,为临床治疗和疾病控制提供参考。方法从上饶市流感监测网中随机选择12株甲型H1N1流感病毒,经核酸提取和one-step RT-PCR扩增M基因及NA基因片段,M基因经双向序列测定,采用DNAStar6.0和MEGA6.0序列分析软件分析M2基因特征以及耐药性位点;NA基因采用限制性内切酶片段长度多态性分析(RFLP)方法分析NA基因第275位是否发生酪氨酸(Y)替代组氨酸(H)的突变。结果 12株新型甲型H1N1流感病毒毒株M2基因31位氨基酸均由丝氨酸(S)突变为天冬酰胺(N);NA基因第275位氨基酸均为组氨酸,未发生变异。结论 12株新型甲型流感病毒毒株均对金刚烷胺类药物耐药,而对神经氨酸酶抑制剂奥司他韦敏感。
Objective To analyze the characteristics of M2 gene and NA gene of Influenza A (H1N1) virus in Shangrao City from 2013 to 2014 and to master the resistance of M2 gene to provide reference for clinical treatment and disease control. Methods A total of 12 influenza A (H1N1) viruses were randomly selected from the influenza surveillance network of Shangrao City. M gene and NA gene fragment were amplified by nucleic acid extraction and one-step RT-PCR. M gene was determined by two-dimensional sequencing. DNAStar6.0 and MEGA6 .0 sequence analysis software was used to analyze the M2 gene characteristics and drug resistance loci; NA gene was analyzed by restriction fragment length polymorphism (RFLP) method to detect whether the tyrosine (Y) Amino acid (H) mutations. Results The amino acids at position 31 of M2 gene of 12 strains of new type A (H1N1) virus were all mutated from serine (S) to asparagine (N). The 275th amino acid of NA gene was histidine, and no mutation occurred. Conclusion All of the 12 new influenza A virus strains are resistant to amantadine drugs and sensitive to neuraminidase inhibitor oseltamivir.