Experiment and dynamic simulation were combined to obtain the loads on bicycle frame.A dynamic model of body-bicycle system was built in ADAMS.Then the body gestures under different riding conditions were captured by a motion analysis system.Dynamic simulation was carried out after the data of body motions were input into the simulation system in ADAMS and a series of loads that the body applied on head tube,seat pillar and bottom bracket were obtained.The results show that the loads on frame and their distribution are apparently different under various riding conditions.Finally,finite element analysis was done in ANSYS,which showed that the stress and its distribution on frame were apparently different when the frame was loaded according to the bicycle testing standard and simulation respectively.An efficient way to obtain load on bicycle frame accurately was proposed,which is significant for the safety of cycling and will also be the basis for the bicycle design of digitalization,lightening and customization.