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本文报道用绵羊中提取的乙型肝炎表面抗原-免疫核糖核酸(HBsAg-iRNA)处理的正常兔血细胞,在加HBsAg培养时,其淋巴细胞参入氚化脱氧胸苷(~3HdT)的量为对照管的2倍,而用佐剂免疫动物提取的核糖核酸(n-RNA)则否。将HBsAg-iRNA用核糖核酸酶(RNase)消化后,则促~3HdT参入作用即消失。如单独用HBsAg-iRNA处理淋巴细胞而不加HBsAg培养,或不用HBsAg-iRNA处理而加HBsAg培养,均不能使~3HdT的参入增加。以上结果表明,HBsAg-iRNA能超越种间界限,传递给淋巴细胞,使其在接触HBsAg时发生特异转化作用,增加~3HdT参入而显示其免疫反应性。
In this paper, normal rabbit blood cells treated with HBsAg-iRNA extracted from sheep were used. The amount of lymphocytes participating in tritiated deoxythymidine (~ 3HdT) in HBsAg-supplemented culture was control Tube 2 times, while animals immunized with adjuvant RNA (n-RNA) is not. After HBsAg-iRNA is digested with RNase, the effect of ~ 3HdT incorporation disappears. Treatment of lymphocytes with HBsAg-iRNA alone without HBsAg or with HBsAg-iRNA plus HBsAg did not lead to an increase in ~ 3HdT. The above results show that HBsAg-iRNA can transcend interspecies boundaries and transmit to lymphocytes to make HBsAg-specific transformation when exposed to HBsAg, and increase the incorporation of ~ 3HdT to show its immunoreactivity.