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利用玉米花培后代与常规自交系组配的杂交组合作为接种材料来源,普遍可以提高愈伤组织诱导率,不同基因型的花培后代互配杂交组合其诱导率更高,最高达49.5％;去掉培养基中甘氨酸,分别加入丙氨酸、丝氨酸、L—谷氨酸、L—赖氨酸、L—羟脯氨酸、L—天冬氨酸等均可提高诱导率,几种氨基酸混合添加效果更好,诱导率比对照高2.6倍;玉米花药液体培养比固体和半固体培养的诱导率高,半固体培养产生的愈伤组织质量比液体培养的好,分化率高;以石油助长剂代替6—糠基氨基嘌呤,对玉米愈伤组织的形成有很好的效果。
The combination of popcorn cultivars and conventional inbred lines as the inoculation material can generally improve the callus induction rate. The induction rate of intercross hybridization of flower genotypes of different genotypes is higher up to 49.5% ; The removal of glycine in culture medium, adding alanine, serine, L-glutamic acid, L-lysine, L-hydroxyproline and L-aspartic acid all can improve the induction rate, The mixed effect was better and the induction rate was 2.6 times higher than that of the control; the induction rate of popcorn liquid culture was higher than that of solid and semi-solid culture, the quality of callus produced by semi-solid culture was better than liquid culture, Extender instead of 6 - furfurylaminopurine, corn callus formation has a good effect.