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目的通过基于液质联用的代谢组学方法,探讨干姜对大鼠尿液内源性物质的影响。方法 20只SD大鼠分为正常对照组和干姜组,干姜组ig给予干姜水提物0.72g·kg-1,每天1次,连续30d,分别于第0,1,8,15,22,29天收集大鼠尿样。经液相色谱分离,三重四极杆质谱对尿液中内源性代谢物进行全扫描分析,采用主成分分析(PCA)法降维,正交信号校正和偏最小二乘法判别分析法(OSC-PLS-DA)分析采集的质荷比和丰度值数据,测定大鼠体内内源性物质含量。结果质荷比和丰度值数据均值主成分分析结果显示,第8天干姜对大鼠尿液中物质代谢影响最大。对第8天数据进行OSC-PLS-DA分析,与正常对照组相比,干姜组大鼠尿液中磷脂酸和神经酰胺-1-磷酸等物质含量显著下降,孕烯醇酮硫酸和鞘磷脂物质含量明显上升。结论干姜对正常大鼠机体代谢有明显的影响,神经酰胺-1-磷酸等10种物质被判定为可能生物标志物。
Objective To investigate the effects of Ginger on endogenous substances in urine of rats by means of metabonomics-based metabonomics. Methods Twenty SD rats were divided into normal control group and Ginger group. The Ginger group was given 0.72 g · kg -1 of Ginger water extract once a day for 30 days, Rat urine samples were collected on days 22 and 29. The total metabolites in the urine were analyzed by liquid chromatography and triple quadrupole mass spectrometry. The principal component analysis (PCA) method was used to reduce the dimension of the endogenous metabolites. Orthogonal signal calibration and partial least squares discriminant analysis (OSC -PLS-DA) to analyze the collected mass-to-charge ratio and abundance data to determine the content of endogenous substances in rats. Results The results of principal component analysis (PCA) of the means of mass-to-charge ratio and abundance data showed that ginger on the 8th day had the most significant effect on the substance metabolism in the urine of rats. OSC-PLS-DA analysis of day 8 data showed that the content of phosphatidic acid and ceramide-1-phosphate in the urine of the ginger group was significantly lower than that of the normal control group. The contents of pregnenolone sulfate and sheath Phospholipid content increased significantly. Conclusion Dry ginger has a significant effect on body metabolism in normal rats. Ten substances, such as ceramide-1-phosphate, have been identified as potential biomarkers.