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以玉米幼苗为材料,研究盐胁迫下其內源NO含量、NR和NOS活性的变化;NOS专一性抑制剂L-NAME和NR非专一性抑制剂NaN3对玉米幼苗內源NO含量的影响;利用激光共聚焦显微技术观测盐胁迫下玉米幼苗根部NO含量的变化及其分布特点。结果表明,盐胁迫下玉米幼苗根尖和叶片中NO含量有猝发现象,NOS活性也随之显著提高,NR活性则显著降低;L-NAME或NaN3均可降低盐胁迫所引起的玉米幼苗NO水平的增加,L-NAME对NO含量的影响比NaN3更显著。推测,NO参与玉米幼苗对盐胁迫的应答,NOS途径是盐胁迫下玉米幼苗內源NO合成的主要途径。
The changes of endogenous NO content, NR and NOS activities under salt stress were studied using maize seedlings as materials. The effects of L-NAME, a specific inhibitor of NOS and NaN3, a non-specific inhibitor of NR, on endogenous NO content in maize seedlings The change of NO content and its distribution in corn seedling roots under salt stress were observed by laser confocal microscopy. The results showed that under salt stress, the content of NO in root tip and leaf of the corn seedling burst, the NOS activity increased significantly and the activity of NR decreased significantly. Both L-NAME and NaN3 could reduce the NO level of maize seedlings caused by salt stress The effect of L-NAME on NO content was more significant than that of NaN3. It is speculated that NO is involved in the response of maize seedlings to salt stress. NOS pathway is the main pathway for endogenous NO synthesis in maize seedlings under salt stress.