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目的建立一种ELISA方法 ,检测患者血清中抗转铁蛋白抗体 ,评价其对子宫内膜异位症诊断的价值。方法用转铁蛋白抗原包被 ,间接ELISA方法检测患者血清中抗转铁蛋白抗体。采集患者血清 ,分 3组 :盆腔内异症组 38例 ,Ⅰ、Ⅱ期 2 0例 ,Ⅲ期 9例 ,Ⅳ期 9例 ;妇科良性疾病对照组 30例 ;正常对照组 4 0例。结果内异症患者血清中抗转铁蛋白抗体阳性率高达 94 .7% ,正常对照组阳性率为 2 .5 % ,良性疾病对照组阳性率为 0 % ,内异症组与两对照组比较差异有显著意义 (P均 <0 .0 1 )。间接ELISA法诊断内异症临床敏感性为 94 .7% ,特异性为 98.6 % ,诊断符合率为 96 .5 %。内异症各组阳性率比较差异无显著意义 (P >0 .0 5 )。结论用转铁蛋白抗原包被的间接ELISA法检测血清中抗转铁蛋白抗体诊断内异症敏感性、特异性强 ,诊断符合率高 ,可试作为内异症辅助诊断及疗效检测的一种非创伤性的指标
Objective To establish an ELISA method for the detection of serum anti-transferrin antibody and evaluate its value in the diagnosis of endometriosis. Methods The serum anti-transferrin antibody was detected by transferrin antigen coating and indirect ELISA. Serum samples were collected and divided into 3 groups: 38 cases of pelvic endometriosis group, 20 cases of stage Ⅰ and Ⅱ, 9 cases of stage Ⅲ and 9 cases of stage Ⅳ; 30 cases of benign gynecological diseases in control group and 40 cases of normal control group. Results The positive rate of anti-transferrin antibody in sera of endometriosis patients was as high as 94.7%, the positive rate of normal control group was 2.5%, the positive rate of benign disease control group was 0%, and the level of endometriosis was significantly higher than that of the two control groups The difference was significant (P <0.01). Indirect ELISA diagnosis of endometriosis clinical sensitivity was 94.7%, specificity was 98.6%, the diagnostic coincidence rate was 96.5%. There was no significant difference in the positive rate of endometriosis between the groups (P> 0.05). Conclusion Indirect ELISA method for the determination of anti-transferrin antibody in serum by indirect ELISA with transferrin antigen coating has high sensitivity and specificity for diagnosing endometriosis and can be used as a diagnostic and therapeutic measure for endometriosis Non-invasive indicators