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目的:探讨血清抑制素B(INHB)在非梗阻性无精子症中的应用及对睾丸精子抽吸结局的预测作用。方法:用回顾性分析方法对52例非梗阻性无精子症(NOA)、12例梗阻性无精子症(OA)及20例正常生育男性,采用双抗夹心ELISA法测定各组血清中INHB水平;采用化学发光法测定各组血清中卵泡刺激素(FSH)、黄体生成素(LH)和睾酮(T)水平。结果:NOA组血清INHB水平明显低于正常生育男性组和OA组,其差异均有统计学意义(P<0.01);血清FSH和LH水平明显高于OA组和正常生育男性组(P<0.01)。NOA组行睾丸抽吸(TESE)获得精子的患者,其血清INHB水平明显高于未获得精子者,其差异有统计学意义(P<0.01);而NOA组行TESE未获得精子的患者,其血清FSH水平明显高于获得精子者,其差异有统计学意义(P<0.01)。比较两组血清中LH和T水平,其差异均无统计学意义(P>0.05)。结论:血清INHB水平可反映睾丸精子发生的情况,能准确预测TESE获取精子的结局。
Objective: To investigate the application of serum inhibin B (INHB) in non-obstructive azoospermia and to predict the outcome of testicular sperm aspiration. Methods: 52 cases of non-obstructive azoospermia (NOA), 12 cases of obstructive azoospermia (OA) and 20 normal fertile men were retrospectively analyzed. The levels of INHB in serum of each group were determined by double-antibody sandwich ELISA The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) in serum of each group were determined by chemiluminescence method. Results: The level of serum INHB in NOA group was significantly lower than that in normal male and female subjects (P <0.01). The levels of serum FSH and LH in serum were significantly higher than those in OA and normal fertile men (P <0.01) ). The level of serum INHB was significantly higher in NOA patients with testicular aspiration (TESE) than those without sperm (P <0.01), while those in NOA group with TESE not sperm Serum FSH levels were significantly higher than those who obtained sperm, the difference was statistically significant (P <0.01). There was no significant difference between the two groups in serum LH and T levels (P> 0.05). Conclusion: The level of serum INHB can reflect the testicular spermatogenesis and accurately predict the outcome of TESE acquisition of sperm.