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慢性类风湿性关节炎(简称RA.)并发贫血发生率很高,有报告达58.1％,其原因尚不全明。本文研究了RA患者淋巴细胞对正常人及RA患者骨髓幼红细胞血红素合成的影响。作者选择RA患者及健康人各13例为研究对象。方法:采骨髓血2—4ml,加到pH7.4的磷酸盐缓冲液5—10ml中。连续2次离心,分离有核细胞层,最后分离出骨髓细胞。另外,采肘静脉血10ml,用pH7.4的Tyrode液稀
Chronic rheumatoid arthritis (RA) has a high incidence of anemia, with a reported rate of 58.1%, the reason for which is not yet fully understood. In this paper, the effects of lymphocytes from patients with RA on heme synthesis of bone marrow erythrocytes in normal and RA patients were studied. The authors selected 13 RA patients and healthy subjects as study subjects. Methods: Bone marrow blood 2-4ml, added to pH7.4 phosphate buffer 5-10ml. Centrifugation was performed twice in succession, the nucleated cell layer was separated, and finally the bone marrow cells were separated. In addition, mining elbow vein blood 10ml, diluted with Tyrode solution pH7.4