目前,对大豆花叶病毒(SMV)种子传毒率的检测主要应用生物学(如苗期症状观察、局部枯斑反应等)和免疫学(如E L I S A.免疫电镜,放射免疫等)两类方法。这两类方法在应用上存在一些问题:如生物学方法测得的是种子传毒率,而免疫学方法测得的是种子带毒率、二者具有不同的意义:以上方法均需逐粒检查种子 At present, the detection of the virus transmission rate of soybean mosaic virus (SMV) is mainly applied to biology (such as observation of seedling symptom, partial dead spot reaction, etc.) and immunology (such as ELIS A. Immunoelectron microscopy, radioimmunoassay, etc.) method. There are some problems in application of these two kinds of methods. For example, the rate of seed transmission is measured by the biological method, and the rate of seed poisoning is measured by the immunological method. The two methods have different meanings: the above methods require grain by grain Check the seeds