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目的 :探讨黄芪、硫酸锌对肠缺血 /再灌注 (I/R)后红细胞 (RBC)膜微粘度的影响并探讨其作用机制。方法 :复制家兔肠I/R损伤模型 ,检测给予黄芪、硫酸锌后肠I/R损伤家兔RBC膜微粘度的变化 ,同时检测RBC超氧化物歧化酶 (SOD)、RBC膜及重要器官组织丙二醛 (MDA)含量及血浆黄嘌呤氧化酶 (XO)活性 ,并与I/R组及假手术组比较。分析膜微粘度与SOD、MDA、XO之间的关系。结果 :黄芪、硫酸锌可使RBC膜微粘度、膜和器官组织MDA及血浆XO活性降低 ,且可防止SOD减少 (P <0 .0 1 )。结论 :黄芪、硫酸锌通过抗脂质过氧化能稳定RBC膜 ,改善RBC膜微粘度 ,进而改善重要器官的血流动力学 ,避免了I/R损伤的进行性加剧
Objective: To investigate the effects of astragalus and zinc sulfate on the microviscosity of red blood cell (RBC) membrane after intestinal ischemia/reperfusion (I/R) and to explore its mechanism of action. METHODS: Rabbit intestinal I/R injury models were duplicated to detect the changes of RBC membrane microviscosity in rabbits after intestine I/R injury after administration of astragalus and zinc sulfate. Simultaneous detection of superoxide dismutase (SOD), RBC membrane and vital organs of RBCs was performed. Malondialdehyde (MDA) content and plasma xanthine oxidase (XO) activity were measured and compared with I/R group and sham operation group. The relationship between membrane microviscosity and SOD, MDA, and XO was analyzed. Results: Astragalus membranaceus and zinc sulphate could decrease the microviscosity, membrane and organ MDA content, and plasma XO activity of RBC membranes, and prevent the reduction of SOD (P < 0.01). Conclusion: Astragalus and zinc sulfate can stabilize RBC membranes by anti-lipid peroxidation, improve micro-viscosity of RBC membranes, improve hemodynamics of vital organs, and avoid progressive I/R injury progression.