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测定尿中三氯乙酸的含量能反映接触三氯乙烯的水平。本文作者报告了一种把三氯乙酸转变为它的甲基酯的快速气相色谱测定法。先用甲苯从尿中提取出三氯乙酸,然后用过量的三氟化硼-甲醇试剂甲基化,用气相色谱测定三氯乙酸的甲基酯。色谱分析的条件是:不锈钢柱,5呎×1/8吋;5%QF-1涂布在Chromosorb W(60～80目)担体上;进样口温度185℃;柱温110℃;鉴定器温度106℃;载气为氮气,流速45毫升/分;电子俘获鉴定器,氚源,90伏,直流;样本体积2微升。所用试剂为3.0
Determination of urinary trichloroacetic acid content can reflect the level of exposure to trichlorethylene. The authors report a rapid gas chromatography method for the conversion of trichloroacetic acid to its methyl ester. Trichloroacetic acid was extracted from the urine with toluene first and then methylated with an excess of boron trifluoride-methanol reagent to determine the trichloroacetic acid methyl ester by gas chromatography. The chromatographic conditions were: stainless steel column, 5 ft x 1/8 inch; 5% QF-1 coated on a Chromosorb W (60-80 mesh) support; inlet temperature 185 ° C .; column temperature 110 ° C; Temperature 106 ° C; carrier gas nitrogen, flow rate 45 ml / min; electron capture detector, tritium source, 90 V, DC; sample volume 2 μl. The reagent used is 3.0