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目的了解陕西省腮腺炎病毒(mumps virus,MuV)流行株的基因型和人群抗体水平。方法采集腮腺炎病人咽拭子标本分离病毒,针对分离到的MuV小疏水蛋白(small hydro-phobic protein,SH)基因316个核苷酸片段进行逆转录-聚合酶链反应扩增,对扩增产物进行序列测定和基因分型;用酶联免疫吸附试验(ELISA)测定健康人群腮腺炎IgG抗体。结果采集咽拭子标本55份,分离出腮腺炎病毒6株,均为F基因型。腮腺炎抗体阳性率为66.08%。IgG抗体含量和阳性率均为<1.5岁组最低,分别为236.88 U/mL和37.04%,以后随年龄增加逐渐升高,性别间差异均无统计学意义(P<0.05)。结论陕西省腮腺炎流行是由F基因型MuV引起,健康儿童腮腺炎免疫水平偏低。
Objective To understand the genotypes and population antibody levels of mumps virus (MIV) strains in Shaanxi Province. Methods The virus was isolated from the pharyngeal swab specimens of mumps patients. The 316 bp fragment of Mu hydrolase (small hydro-phobic protein) was amplified by reverse transcription-polymerase chain reaction (PCR) The products were sequenced and genotyped. The mumps IgG antibodies in healthy population were determined by enzyme linked immunosorbent assay (ELISA). Results 55 samples of throat swabs were collected and 6 mumps viruses were isolated, all of which were F genotypes. Mumps antibody positive rate was 66.08%. IgG antibody levels and positive rates were the lowest in the group <1.5 years old, respectively 236.88 U / mL and 37.04%, then increased with increasing age, sex differences were not statistically significant (P <0.05). Conclusions The prevalence of mumps in Shaanxi Province is caused by the F genotype MuV, and the immunization level of mumps in healthy children is low.