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目的制备80%至10%的波动氧所致SD新生大鼠视网膜病变的模型。方法新生SD大鼠随机分成两组,模型组(n=12)置于氧箱中,80%至10%的波动氧24 h交替,持续7天后转入空气中饲养5天。对照组(n=12)置于空气中。应用腺苷二磷酸酶染色组织化学法进行视网膜铺片观察视网膜血管改变,眼球切片HE染色观察突破视网膜内界膜的内皮细胞核,免疫组织化学法检测视网膜血管内皮生长因子(VEGF)表达。对两组进行比较,判断波动氧诱导模型的可靠性。结果与对照组相比,模型组新生大鼠视网膜周边血管走形迂曲,神经节细胞排列紊乱,细胞数目减少。模型组视网膜内皮细胞核数目较对照组明显升高,差异有统计学意义[(31.2±6.2)比(1.5±1.0),P<0.01]。模型组VEGF在视网膜神经节层、内核层、外丛状层及色素细胞层均有强表达,对照组仅在神经节细胞层、内核层有较弱表达。结论吸入高幅波动氧可导致新生大鼠视网膜血管增生性病变,血管增生性视网膜组织中VEGF表达明显增强,证实80%至10%的波动氧诱导SD新生大鼠视网膜病变模型可靠。
Objective To prepare a model of retinopathy of SD neonatal rats induced by fluctuating oxygen of 80% to 10%. Methods Newborn SD rats were randomly divided into two groups. The model group (n = 12) was placed in an oxygen tank. The fluctuating oxygen of 80% to 10% was alternated for 24 h and then kept in the air for 5 days after 7 days. The control group (n = 12) was placed in the air. The retinal vascular changes were observed by retinal staining using histochemical method with adenosine diphosphate staining. Endothelial cell nucleus of the inner limiting membrane of the retina was observed by HE staining and the expression of vascular endothelial growth factor (VEGF) was detected by immunohistochemistry. The two groups were compared to determine the reliability of the wave-induced oxygen model. Results Compared with the control group, the blood vessels around the retina of the model group were tortuous, the ganglion cells were disordered and the number of cells decreased. The number of retinal endothelial cells in the model group was significantly higher than that in the control group ([(31.2 ± 6.2) vs (1.5 ± 1.0), P <0.01]. In the model group, VEGF was strongly expressed in the retinal ganglion layer, inner nuclear layer, outer plexiform layer and pigment cell layer, while the control group only expressed weakly in the ganglion cell layer and inner nuclear layer. Conclusion Inhalation of fluctuating oxygen can lead to retinal vascular proliferative lesions in neonatal rats, and the expression of VEGF in vascular proliferative retina significantly increased. It is confirmed that 80% to 10% of fluctuating oxygen can induce retinopathy of SD neonatal rat model.