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【目的】获得具有产ACC、IAA,铁载体,能固氮或解磷的潜在促生菌株。【方法】通过稀释涂布的方法,从麻疯树根际土壤中分离得到98株细菌,从中选取28株以产1-氨基环丙烷-1-羧酸(ACC)脱氨酶为主要促生指标进行筛选,同时检测了其产吲哚乙酸(IAA)、固氮、解磷及铁载体等促生指标的能力。【结果】结果显示,46%的菌株能产ACC脱氨酶,其含量最高可达到128.308μmolα-KA/(mg.h),68%的菌株能产生IAA,54%的菌株有固氮的能力,32%的菌株有解磷的能力。少量菌株同时具有产ACC脱氨酶、IAA,固氮,解磷等能力。挑选代表性菌株进行16S rRNA序列分析,这些菌株属于芽孢杆菌属(Bacillus)、节杆菌属(Arthrobacter)、假单胞菌属(Pseudomonas)和产碱杆菌属(Advenella)等8个属,其中多数菌株(50%)属于芽孢杆菌属,系统发育分析表明菌株KLBMP 4817、KLBMP 4821和KLBMP 4824为窄食单胞菌属(Stenotrophomonas)和类芽孢杆菌属(Paenibacillus)的潜在新种。【结论】攀枝花麻疯树根际土壤细菌中含有丰富的遗传多样性,且存在大量的促生菌株。其中,菌株KLBMP 4804产ACC脱氨酶含量最高。菌株KLBMP 4820产IAA含量最显著。
【Objective】 Potential promoters of ACC, IAA, iron carriers, nitrogen-fixing or phosphate-solubilizing strains were obtained. 【Method】 98 strains of bacteria were isolated from the rhizosphere soil of J. curcas by means of dilution coating. 28 strains were selected to produce 1-aminocyclopropane-1-carboxylic acid (ACC) Indicators were screened at the same time its production of indole acetic acid (IAA), nitrogen fixation, phosphorus and iron vector and other indicators of the ability to promote birth. 【Result】 The results showed that 46% of the strains could produce ACC deaminase with the highest content of 128.308μmolα-KA / (mg.h), 68% strains could produce IAA, 54% 32% of the strains have the ability to solve phosphorus. A small number of strains also have the production of ACC deaminase, IAA, nitrogen fixation, phosphorus and other capabilities. Representative strains were selected for 16S rRNA sequence analysis. These strains belong to 8 genera, such as Bacillus, Arthrobacter, Pseudomonas and Advenella, The strain (50%) belongs to the genus Bacillus and phylogenetic analyzes show that the strains KLBMP 4817, KLBMP 4821 and KLBMP 4824 are potential new species of Stenotrophomonas and Paenibacillus. 【Conclusion】 The rhizosphere soil of Panzhihua jade tree is rich in genetic diversity, and there are a lot of growth-promoting bacteria. Among them, strain KLBMP 4804 produced the highest content of ACC deaminase. The strain KLBMP 4820 produced the most significant IAA content.