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目的 探讨慢性肝病及肝癌组织中PCNA表达及其生物学意义。方法 应用ABC免疫组化方法研究 2 0例慢性肝病 ( 7例CPH、8例CAH、5例LC)和 39例原发性肝癌 (肝细胞性肝癌 34例 ,胆管细胞性肝癌 5例 )组织中PCNA表达。结果 正常肝组织PCNA阳性细胞表达率均小于 3%。在慢性肝病中 ,CAH( 7/ 8)和LC( 5/ 5)组织中的PCNA阳性细胞表达率显著高于CPH( 3/ 7) (P <0 0 5) ,肝癌组织中PCNA阳性强级表达明显增高 ,且与肝癌的分化程度、淋巴结转移有关。结论 PCNA检测可作为慢性肝病发展或向恶性转化的重要预测因子 ,对预测肝癌的发生及早期发现肝癌有重要意义 ,并有且肝癌恶性程度及预后的判断。
Objective To investigate the expression of PCNA in chronic liver disease and hepatocellular carcinoma and its biological significance. Methods ABC immunohistochemical method was used to study the expression of 20 chronic hepatitis (7 CPH, 8 CAH, 5 LC) and 39 primary hepatocellular carcinoma (hepatocellular carcinoma, 5 cholangiocarcinoma) PCNA expression. Results The positive rates of PCNA positive cells in normal liver tissue were less than 3%. In chronic liver disease, the positive rate of PCNA positive cells in CAH (7/8) and LC (5/5) tissues was significantly higher than that in CPH (3/7) (P <0.05) The expression was significantly increased, and with the degree of differentiation of liver cancer, lymph node metastasis. Conclusion The detection of PCNA can be used as an important predictor of the development of chronic liver disease or malignant transformation. It is important for predicting the occurrence of hepatocellular carcinoma and early detection of hepatocellular carcinoma (HCC), and the judgment of malignant degree and prognosis of HCC.