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目的联合观测HIF-1α、COX-2在胃肠道腺癌中的表达及其临床意义。方法以经病理证实的37例为胃肠道腺癌组,其淋巴结转移组织37例作为淋巴结转移癌组,正常胃肠道黏膜组织37例作为正常胃肠道黏膜组,采用链霉素-生物素(S-P)免疫组化技术,分别检测三组组织中HIF-1α、COX-2的表达。采用Wilcoson秩和检验进行组间比较。结果胃肠道腺癌组和淋巴结转移癌组中HIF-1α阳性表达率均明显高于正常胃肠道黏膜组织(P<0.05);淋巴结转移癌组及胃肠道腺癌组组织中的HIF-1α、COX-2阳性表达率之间差异无统计学意义(P>0.05)。结论 HIF-1α、COX-2在胃肠道腺癌及其淋巴结转移癌的组织中表达水平高于正常胃肠道黏膜,它们共同参与了肿瘤的发生、发展、浸润、转移,联合检测上述标志物有一定的临床及病理意义。
Objective To observe the expression of HIF-1α and COX-2 in gastrointestinal adenocarcinoma and its clinical significance. Methods Totally 37 cases of gastrointestinal adenocarcinoma were confirmed by pathology, 37 cases of lymph node metastasis as lymph node metastasis group and 37 cases of normal gastrointestinal mucosa as normal gastrointestinal mucosa group. Streptomycin - (SP) immunohistochemistry to detect the expression of HIF-1α and COX-2 in three groups respectively. Wilcoxon rank sum test was used to compare groups. Results The positive rates of HIF-1α in gastrointestinal adenocarcinoma group and lymph node metastasis group were significantly higher than those in normal gastric mucosa (P <0.05). The positive rates of HIF-1α in lymph node metastasis group and gastrointestinal adenocarcinoma group There was no significant difference in the expression of COX-1α and COX-2 (P> 0.05). Conclusions The expression of HIF-1α and COX-2 in gastrointestinal adenocarcinoma and lymph node metastasis tissues is higher than that in normal gastrointestinal mucosa, and they are involved in the occurrence, development, invasion, metastasis of tumor and joint detection of the above markers There is a certain clinical and pathological significance.