【摘 要】
：
目的 观察miRNA-26b(miR-26b)在野生型C57/BL6J小鼠和瘦素基因遗传性缺陷ob/ob小鼠组织中的表达特征,以及瘦素对3T3-L1成熟脂肪细胞中miR-26b表达的调控.方法 取16周龄C57/BL6J小鼠和ob/ob小鼠的心脏、肝脏、脾、胰、肾脏、肺、小肠、骨骼肌、白色脂肪组织和棕色脂肪组织,采用实时荧光定量聚合酶链反应(qRT-PCR)技术检测小鼠各种组织中miR-26b表达水平;采用100 ng/mL的瘦素干预3T3-L1成熟脂肪细胞16 h,采用qRT-PCR技术检测细胞中miR
【机 构】
：
江苏省淮安市第三人民医院检验科,江苏淮安 223001
论文部分内容阅读
目的 观察miRNA-26b(miR-26b)在野生型C57/BL6J小鼠和瘦素基因遗传性缺陷ob/ob小鼠组织中的表达特征,以及瘦素对3T3-L1成熟脂肪细胞中miR-26b表达的调控.方法 取16周龄C57/BL6J小鼠和ob/ob小鼠的心脏、肝脏、脾、胰、肾脏、肺、小肠、骨骼肌、白色脂肪组织和棕色脂肪组织,采用实时荧光定量聚合酶链反应(qRT-PCR)技术检测小鼠各种组织中miR-26b表达水平;采用100 ng/mL的瘦素干预3T3-L1成熟脂肪细胞16 h,采用qRT-PCR技术检测细胞中miR-26 b表达水平.结果 ob/ob小鼠的体质量、血糖、甘油三酯及总胆固醇水平明显高于C57/BL6J小鼠,差异均有统计学意义(P<0.05).miR-26b在C57/BL6J小鼠心脏、脂肪、胰组织中表达水平较高,而在小肠、肾脏、骨骼肌组织中表达水平较低.与C57/BL6J小鼠比较,miR-26 b在ob/ob小鼠心脏、胰、白色脂肪组织中表达水平明显下降,而在肺、骨骼肌、肾脏及小肠组织中表达水平升高,差异有统计学意义(P<0.05);在小鼠3T3-L1成熟脂肪细胞中,瘦素可明显下调miR-26b表达.结论 ob/ob小鼠各种组织中miR-26 b表达呈现不同的变化特征,miR-26 b是一种与机体糖脂代谢功能密切相关的miRNA.
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