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目的采用HPLC法测定柔肝解毒胶囊中的绿原酸。方法采用Diamonsil C18色谱柱(200 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸溶液(10:90),柱温为室温,流速1.2 mL.min-1,检测波长327 nm。结果回归方程为:Y=3.031×103X+1.042×104(r=0.9998),绿原酸90~800 ng与峰面积的线性关系良好,平均回收率为100.86%,RSD=1.08%(n=6),精密度和重复性的RSD分别为0.41%、1.31%。结论所建方法分离效能高、灵敏准确、重复性好,可作为质控方法 。
Objective To determine the chlorogenic acid in Rougan Jiedu capsule by HPLC. Methods Diamonsil C18 column (200 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-0.4% phosphoric acid solution (10:90). The column temperature was at room temperature and the flow rate was 1.2 mL.min-1. The detection wavelength was 327 nm. Results The regression equation was: Y = 3.031 × 103X + 1.042 × 104 (r = 0.9998). The linear relationship between the peak area and the area of 90 ~ 800 ng of chlorogenic acid was good with the average recovery of 100.86% and RSD of 1.08% ), RSD of precision and repeatability were 0.41% and 1.31% respectively. Conclusion The established method has the advantages of high efficiency, sensitiveness and accuracy, good reproducibility and can be used as a quality control method.