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目的探索硫酸盐还原菌的检测方法,为基层部队实验室检测提供参考。方法采用化学除氧和指示剂结合的方法,在培养基中加入氧化还原剂,以使在密闭的环境中自行除氧,从而建立起无氧环境,使厌氧菌获得生长繁殖环境。同时利用硫酸盐还原菌生长繁殖过程中产生的硫化氢与硫酸亚铁生成黑色硫化亚铁,从而指示硫酸盐还原菌细菌的存在。结果该检测反应比较敏感,阴、阳对照样本的检出率分别为0%和100%。样本检测结果显示,长期停用的自备水井、生活污水和含燃料污水中均有硫酸盐还原菌存在。结论硫酸盐还原菌普遍存在于环境中,因其能产生硫化氢,不仅会污染水源、影响水质,还会对环境产生危害,对其实施检测,意义重大。
Objective To explore the detection methods of sulfate-reducing bacteria and provide a reference for laboratory detection of grassroots units. Methods By combining chemical deoxygenation and indicator, the redox agent was added to the medium to deoxygenate itself in the closed environment to establish an anaerobic environment and enable the anaerobic bacteria to obtain the growth and reproduction environment. At the same time, the use of H2S and ferrous sulfate produced during the growth and reproduction of sulfate-reducing bacteria to produce black ferrous sulfide indicates the presence of bacteria that are sulfate-reducing bacteria. Results The test was sensitive, and the detection rates of negative and positive control samples were 0% and 100% respectively. The results of the sample test showed that sulfate-reducing bacteria existed in the self-provided wells, domestic sewage and fuel-containing sewage which were suspended for a long time. Conclusion Sulfate reducing bacteria are commonly found in the environment. Because they produce hydrogen sulfide, they not only pollute the water and affect the water quality, but also cause harm to the environment. It is of great significance to carry out the test.