目的 :建立六一散中甘草酸单铵的含量测定方法。方法 :超声提取 ,HPLC测定六一散中甘草酸单铵的含量。使用C18柱 ,甲醇 含 3 %冰醋酸的 0 .2mol/L醋酸铵溶液 (6 2∶38)为流动相 ,检测波长为 2 5 0nm。结果 :此方法线性关系良好 ,平均加样回收率为 99.4%。结论 :本方法分离良好 ,可用于六一散的质量控制 Objective : To establish a method for the determination of monoammonium glycyrrhizinate in Liuyi Powder. Methods: Ultrasonic extraction and HPLC were used to determine the content of monoammonium glycyrrhizinate in Liuyi Powder. Using a C18 column, methanol containing 3% glacial acetic acid in a 0.2 mol/L ammonium acetate solution (6:38) was used as the mobile phase and the detection wavelength was 250 nm. Results : This method has a good linear relationship with an average recovery of 99.4%. Conclusion : This method is well separated and can be used for quality control of Liuyisan