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为建立可靠的大鼠胚胎移植受胚模型，使适用于着床前染毒致畸的机制研究，本文比较输精管结扎绝育和氯丙二醇灌胃绝育雄鼠制备假孕受胚鼠的优劣。结果表明以氯丙二醇绝育雄鼠制备假孕受胚鼠，可简化实验步骤，并明显提高移植胚胎着床率。该法用于着床前胚胎阿司匹林染毒致畸机制研究，在受胚鼠妊娠末期可见胚胎吸收率和畸形率呈剂量依赖性增高，与着床前胚胎的胚泡化率呈负相关，提示该法可成功地用于药源性着床前胚胎异常，排除母体对胎仔发育的影响因素。
In order to establish a reliable embryo transfer embryo model and make it suitable for the mechanism of pre-implantation teratogenicity, this article compares the advantages and disadvantages of vasectomy and chlorpromazine intragastric sterilization of male embryos. The results showed that the preparation of pseudopregnant embryos by maleic anhydride with chloropropylene glycol could simplify the experimental procedure and significantly improve the implantation rate of the transplanted embryos. This method was used to study the mechanism of preimplantation aspirin exposure to teratogenicity. In the end of pregnancy, the rate of embryo absorptivity and malformation rate showed a dose-dependent increase, which was negatively correlated with the rate of preimplantation embryo blastocyst The method can be successfully used for drug-induced preimplantation embryo abnormalities, excluding maternal impact on fetal development factors.