论文部分内容阅读
目的建立五参安神胶囊的质量标准。方法采用TLC法对石菖蒲、首乌藤、五味子进行定性鉴别;采用HPLC法测定五味子醇甲的含量,以十八烷基硅烷键合硅胶为填充剂,以甲醇-水(70∶30)为流动相,流速为1.0 ml·min~(-1),柱温为30℃,检测波长为250 nm。结果五味子、石菖蒲、首乌藤的TLC图均斑点清晰,阴性无干扰;五味子醇甲在1.24~39.6μg·ml~(-1)范围内线性关系良好(r=0.9999),平均回收率为100.89%,RSD为1.61%(n=9)。结论研究方法准确可靠,重复性好,可用于五参安神胶囊的质量控制。
Objective To establish the quality standard of Wushen Anshen Capsule. Methods The TLC method was used for the qualitative identification of Acorus gramineus, Shou Wu vine and Schisandra chinensis. The content of Schisandra chinensis A was determined by HPLC. The octadecylsilane bonded silica gel was used as filler and the methanol-water (70:30) as The mobile phase was at a flow rate of 1.0 ml · min ~ (-1). The column temperature was 30 ℃ and the detection wavelength was 250 nm. Results The TLC images of Schisandra chinensis, Acorus calamus, and Amomum villosum were both clear spots and negative without interference. The calibration curve was linear in the range of 1.24 ~ 39.6μg · ml -1 (r = 0.9999) and the average recovery was 100.89%, RSD 1.61% (n = 9). Conclusion The research method is accurate and reliable with good repeatability and can be used for quality control of Wushen Anshen Capsule.