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根据小鼠的体质量,以不同剂量经口连续染毒纳米氧化锌,90d后摘眼球取血,对血脂、血糖、肝脏抗氧化指标进行检测。结果显示:对照组、80mg/kg剂量组小鼠血糖显著高于160mg/kg剂量组(P<0.05)。对照组、80mg/kg剂量组小鼠血糖极显著高于40mg/kg剂量组(P<0.01);40,80 mg/kg剂量组小鼠血脂含量显著高于对照组(P<0.05),160mg/kg剂量组小鼠血脂含量极显著高于对照组(P<0.01);40,160 mg/kg剂量组小鼠血清总胆固醇含量显著高于对照组(P<0.05);40mg/kg剂量组小鼠血清低密度脂蛋白含量显著高于对照组、80mg/kg剂量组(P<0.05),各组间血清高密度脂蛋白含量无显著差异;80,160mg/kg剂量组小鼠肝脏过氧化氢酶活性显著高于对照组、40mg/kg剂量组(P<0.05),80mg/kg剂量组小鼠肝脏过氧化氢酶活性极显著高于40 mg/kg剂量组(P<0.01);40,80mg/kg剂量组小鼠肝脏Cu-Zn SOD活性显著高于对照组(P<0.05);160 mg/kg剂量组小鼠肝脏抑制羟自由基能力显著低于对照组、40mg/kg剂量组及80mg/kg剂量组(P<0.05);随染毒剂量增加,小鼠肝脏MDA含量增加,但各组间含量差异不显著;随染毒剂量增加,小鼠肝脏谷胱甘肽过氧化物酶活性提高;但各组间活性差异不显著。各组间肝脏H2O2含量、总SOD活性无显著性差异。病理学检查发现,大脑细胞存在噬神经现象,脾脏红髓与白髓的界限不清晰;肺泡壁增厚,局部炎性细胞浸润,曲精小管间的间隙增大,生精细胞排列紊乱、脱落。结果表明:经口连续染毒纳米氧化锌90d能影响雄性小鼠肝脏抗氧化系统,导致小鼠糖、脂代谢紊乱,引起高脂血症,纳米氧化锌对雄性小鼠具有神经毒性及生殖毒性。
According to the body weight of mice, nano zinc oxide was continuously and orally administered at different doses for 90 days, then blood was taken from the eyeballs and blood lipids, blood glucose and liver antioxidants were measured. The results showed that in the control group, the blood glucose in the 80mg / kg group was significantly higher than that in the 160mg / kg group (P <0.05). In the control group, the blood glucose of the 80 mg / kg dose group was significantly higher than that of the 40 mg / kg dose group (P <0.01), while the lipid level of the 40 and 80 mg / kg dose groups was significantly higher than that of the control group (P <0.05) (P <0.01). The levels of serum total cholesterol in the 40 and 160 mg / kg groups were significantly higher than those in the control group (P <0.05), while those in the 40 mg / kg group The content of serum low density lipoprotein in serum was significantly higher than that in control group and 80mg / kg dose group (P <0.05), but there was no significant difference in serum high density lipoprotein between the two groups. The catalase activity in liver of 80 and 160mg / (P <0.05). Compared with the control group, the catalase activity of the liver in the 40 mg / kg dose group was significantly higher than that in the 40 mg / kg dose group (P <0.01) kg, the Cu-Zn SOD activity in the liver of mice was significantly higher than that of the control group (P <0.05); the ability of inhibiting the hydroxyl radical in the liver of 160 mg / kg group was significantly lower than that of the control group, 40mg / kg dose group and 80mg / kg dose group (P <0.05). With the increase of the dose, the content of MDA in the liver of mice increased, but there was no significant difference among the groups. With the increase of the dose, the hepatic glutathione peroxidase activity increased However, there was no significant difference in activity between groups. There was no significant difference in the contents of H2O2 and total SOD between the groups. Pathological examination found that the brain cells have the phenomenon of autopsy, the spleen of red pulp and white pulp of the boundaries is not clear; alveolar wall thickening, local inflammatory cell infiltration, interstitial tubules increased gap, spermatogenic cells arranged disorder, off . The results showed that oral administration of nano zinc oxide continuously for 90 days could affect the antioxidant system of the liver of male mice, resulting in disorder of glucose and lipid metabolism in mice and causing hyperlipidemia. Nano zinc oxide was neurotoxic and reproductive toxic to male mice .