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目的研究不同产地太子参的基因差异表达,分离并鉴定相关差异基因。方法采用反转录双链DNA扩增片段长度多态性技术分析国内4个主产地太子参差异表达基因片段。结果筛选6个引物组合进行扩增,从不同产地太子参中获得34条差异表达转录衍生片段(trivially distributed file system,TDFs),对差异片段进行克隆、序列测定,得到21个TDFs核苷酸序列。经过BLASTX程序比对,15个TDFs有其对应的显著同源序列,其中7个TDFs为已知功能的蛋白,这些蛋白主要参与植物的生长发育、抵御病虫害、提高植物抗非生物胁迫的能力。结论利用c DNA-AFLP技术鉴定出不同产地太子参的差异基因,为揭示太子参药材品质形成的分子机制提供基础资料。
Objective To study the differential gene expression of Pseudostellaria heterophylla in different habitats and to isolate and identify related differential genes. Methods Reverse transcriptional double-stranded DNA amplification fragment length polymorphism (PCR-RFLP) was used to analyze the differentially expressed genes of Pseudostellaria heterophylla in four major producing areas in China. Results Six primer combinations were screened for amplification. Twenty-four differentially expressed TDFs were obtained from different regions of Pseudostellaria heterophylla, and the fragments were cloned and sequenced to obtain 21 TDFs nucleotide sequences . Fifteen TDFs have their corresponding homologous sequences through BLASTX program. Among them, seven TDFs are known functions. These TDFs are mainly involved in plant growth and development, resist pests and diseases, and enhance the ability of plants to resist abiotic stress. Conclusion The differential gene of Radix Pseudostellaria heterophylla from different areas was identified by c DNA-AFLP technique, which provided the basic information for revealing the molecular mechanism of Radix Polygoni multiflori.