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目的了解仙居县结核分枝杆菌临床分离株不同基因型的分布情况,探讨该县结核病分子流行病学特征。方法收集2011年4月-2012年3月仙居县分离的80株结核分枝杆菌,采用15位点VNTR方法进行基因分型,用Bio Numerics 5.0软件对结果进行聚类分析。结果 15个VNTR位点中,MIRU26和Mtub21(HGI=0.827)多态性较高,ETRC(HGI=0.165)和ETRB(HGI=0.292)多态性较差;15位点VNTR分型方法的HGI指数为0.998。经Bio Numberics5.0软件聚类分析,可将检测到的78个基因型分为8个基因群(Ⅰ群~Ⅷ群),各群所含基因型分别为Ⅰ群7个,占8.97%;Ⅱ群8个,占10.26%;Ⅲ群43个,占55.13%;Ⅳ群5个,占6.41%;Ⅴ群2个,占2.56%;Ⅵ群7个,占8.97%;Ⅶ群4个,占5.13%;Ⅷ群2个,占2.56%。结论仙居县流行的结核分枝杆菌VNTR基因存在明显多态性,至少有8个VNTR基因群,主要流行群为Ⅲ群。
Objective To understand the distribution of different genotypes of clinical isolates of Mycobacterium tuberculosis in Xianju County and to explore the molecular epidemiological characteristics of tuberculosis in this county. Methods Eighty Mycobacterium tuberculosis isolated in Xianju County from April 2011 to March 2012 were collected and genotyped by 15 locus VNTR. The results were clustered by Bio Numerics 5.0 software. Results The polymorphisms of MIRU26 and Mtub21 (HGI = 0.827) were higher among 15 VNTR loci. The polymorphisms of ETRC (HGI = 0.165) and ETRB (HGI = 0.292) The index is 0.998. According to the clustering analysis by BioNumberics5.0 software, 78 genotypes could be divided into 8 gene groups (Ⅰ to Ⅷ). The genotypes of each genotype were 7, accounting for 8.97%. 8 in group Ⅱ, accounting for 10.26%; group Ⅲ in 43, accounting for 55.13%; group Ⅳ in 5, accounting for 6.41%; groupⅤ in 2, accounting for 2.56%; group VI in 7, accounting for 8.97% Accounting for 5.13%; Ⅷ group 2, accounting for 2.56%. Conclusion The prevalence of Mycobacterium tuberculosis VNTR gene in Xianju County is obviously polymorphic. There are at least 8 VNTR gene groups and the main epidemic group is Group Ⅲ.