论文部分内容阅读
Ｍ＿９的杂种幼胚需７０ｄ以上的发育期才具备发芽能力；在ＭＳ＋ＢＡ２．０ｍｇ·ｌ￣（－１）＋ＧＡ３２．０ｍｇ·ｌ￣（－１）琼脂培养基上，８０ｄ胚龄的幼胚成苗率达５０．１％；摘除子叶促进了幼胚的成苗；６０ｄ、４℃低温处理可将幼胚成苗率由１１．７％提高到６８．９％。
M_9 hybrid embryos need to germinate more than 70d developmental stages; MS + BA2.0mg · l ~ (-1) + GA32.0mg · l ~ (-1) agar medium, 80d embryonic age immature embryos The rate of 50.1% was achieved. Cotyledon explants promoted the seedling formation of immature embryos. After 60 ℃ and 4 ℃ low temperature treatment, the seedling rate of immature embryos increased from 11.7% to 68.9%.