论文部分内容阅读
低倍率组织切片的摄影通常使用低倍放大摄影装置或光学显微镜。两者成像不同,低倍放大摄影装置是一次成像的像,而光学显微镜是二次成像的像,由于光学特性的不同,出现成像的差异。本研究是通过低倍放大摄影装置和光学显微镜对组织切片分别拍摄,用光学理论加以分析对比,以便在实践中选择应用。倍率及摄影装置(低倍放大摄影装置与光学显微镜)的选择是基于成像是否有良好的解像度,由实验结果得出,低倍放大摄影装置适合于×5以下的拍摄,细微部可以得到良好的显现。×1与×2低倍率显微镜物镜的球面差较大,不适于低倍率摄影。但是,放大机的再次成像时,可取底片中心的60％～70％制成照片,会消除一些由于低倍物镜球面差引起的不足。在摄影装置的选择使用时,还应注意物镜的光圈数。
Low-magnification tissue sections are usually taken using low-magnification photographic equipment or optical microscopy. The two different imaging, low-magnification photographic equipment is an imaging of the image, and optical microscope is a secondary imaging of the image, due to the different optical properties, imaging differences appear. This study is through low magnification photographic equipment and optical microscopy of tissue sections were taken, the optical theory to analyze and compare, in order to choose the application in practice. The magnification and the selection of the photographing apparatus (low power magnification photographing apparatus and optical microscope) are based on whether the imaging has a good resolution or not. From the experimental results, the low magnification photographing apparatus is suitable for photographing less than 5, appear. × 1 and × 2 low magnification microscope objective lens spherical difference is large, not suitable for low magnification photography. However, the magnification of the re-imaging, preferably 60% to 70% of the center of the film made of photos, will eliminate some of the shortcomings due to low power spherical aberration caused. In the selection of photographic equipment used, you should also pay attention to the objective lens aperture.