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目的 探讨膀胱移行细胞癌 (TCCB)组织中端粒酶活性和增殖细胞核抗原 (PCNA)的表达关系及临床意义 方法 采用TRAP ELISA法及免疫组化方法测定 5 8例TCCB组织的端粒酶活性和PCNA表达情况。 结果 TCCB的端粒酶阳性率为 86 .2 % (5 0 / 5 8) ,不同病理分级或临床分期之间差别无显著性意义 (P >0 .0 5 ) ;PCNA表达强度与分级或分期呈正相关 (P <0 .0 5 ) ;不同PC NA表达强度的TCCB样品之间 ,端粒酶活性差别有显著性意义 (P <0 .0 5 ) ,二者呈正相关。 结论 TCCB组织中端粒酶活性与PCNA表达有较好的协同性 ,二者联合检测对TCCB的诊断治疗和估计预后有重要意义。
Objective To investigate the relationship between telomerase activity and proliferating cell nuclear antigen (PCNA) expression in transitional cell carcinoma of the bladder (TCCB) and its clinical significance. Methods TRAP ELISA and immunohistochemistry were used to detect the telomerase activity in 58 TCCB tissues, PCNA expression. Results The positive rate of telomerase in TCCB was 86.2% (50/58). There was no significant difference between the different pathological grades or clinical stages (P> 0.05). The expression of PCNA was correlated with the grade or stage (P <0.05). There was a significant difference in telomerase activity between TCCB samples with different PCNA expression intensity (P <0.05), which showed a positive correlation between them. Conclusions There is a good synergistic effect between telomerase activity and PCNA expression in TCCB tissues. The combination of the two assays is of great significance in the diagnosis and treatment of TCCB and in the estimation of prognosis.