RNA干扰现象自从被发现以来,广泛应用于基因功能方面的研究。应用转基因技术,将表达shRNA的“表达元件”整合到动物基因组中,产生转基因RNA干扰动物,该动物模型体内将不同程度地减少甚至缺失目的基因的表达,为基因功能研究、疾病模型构建以及基因治疗提供了新的平台。本文综述了构建转基因RNA干扰动物的方法,构建条件转基因RNA干扰动物的载体系统以及转基因RNA干扰动物的应用前景。 Since its discovery, RNA interference has been widely used in gene function research. Using genetically modified technology, the “expression element” expressing shRNA is integrated into the animal genome to generate transgenic RNA interfering animals. The animal model will reduce or even eliminate the expression of the target gene in vivo to a certain extent, As well as gene therapy provides a new platform. This review summarizes the methods of constructing transgenic RNA-interfering animals, the construction of vector systems for transgenic RNA-interfering animals, and the potential applications of transgenic RNA-interfering animals.