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A novel method for the analysis of composition distribution of titanium alloys over a large area (64mm × 72 mm) was investigated by exploring the original position statistic distribution based on spark spectrum (OPA-SS) in Ti-6.5Al-1.0Cr-0.5Fe-6.0Mo-3.0-Sn-4.0Zr titanium alloy.The results showed that OPA-SS could characterize the distribution of elements in different positions on the titanium alloy.The macrosegregation of Sn was the most pronounced,with a statistic segregation degree higher than 18％;the macrosegregation of Mo followed with a statistic segregation degree of 10％;the macrosegregation of Al and Fe was relatively milder,lower than 8％.The main reason for the macrosegregation state of the as-cast Ti-6.SAl-1.0Cr-0.SFe-6.0Mo-3.0Sn-4.0Zr alloy can be the solute redistribution during liquid solidification and the diffusion rate of each element in the solid phase.