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目的:观察安子合剂对ACA阳性先兆流产患者CD4+CD25+FOXP3+Treg细胞免疫调节的影响。方法:选取ACA阳性先兆流产患者为治疗组(27例),给服中药安子合剂,125ml,2次/日,连服4周;选取正常孕妇为对照组(15例)。检测患者治疗前后及正常孕妇外周血CD4+CD25+FOXP3+Treg细胞的比例,并测定患者治疗前后血清ACA指标、B超检查胚胎发育情况等。结果:治疗组患者治疗前CD4+CD25+FOXP3+Treg细胞的比例显著低于正常妊娠对照组(P<0.01);治疗后,其细胞比例显著高于治疗前(P<0.01)。27例患者中20例ACA阳性的转阴率达85.00%,7例ACA定量滴度较治疗前显著下降(P<0.01)。保胎的成功率达92.59%。结论:ACA阳性先兆流产患者外周血CD4+CD25+FOXP3+Treg细胞的比例低于正常妊娠妇女,存在免疫调节异常;安子合剂治疗ACA阳性先兆流产的作用机理之一与增加CD4+CD25+FOXP3+Treg细胞的比例、调节妊娠期机体免疫功能有关。
Objective: To observe the effect of Anzizi mixture on immune regulation of CD4 + CD25 + FOXP3 + Treg cells in patients with ACA-positive threatened abortion. Methods: The patients with ACA positive threatened abortion were selected as the treatment group (n = 27). The Chinese medicine Yazi Mixture was given to 125 ml twice daily for 4 weeks. The normal pregnant women were selected as the control group (n = 15). The proportion of CD4 + CD25 + FOXP3 + Treg cells in peripheral blood of patients before and after treatment and normal pregnant women were measured. Serum ACA indexes before and after treatment and the embryonic development of B ultrasound examination were determined. Results: The proportion of CD4 + CD25 + FOXP3 + Treg cells before treatment in treatment group was significantly lower than that in normal pregnancy control group (P <0.01). After treatment, the proportion of CD4 + CD25 + FOXP3 + Treg cells in treatment group was significantly higher than that before treatment (P <0.01). The ACA positive rate of 20 cases in 27 patients was 85.00%. The quantitative titer of ACA in 7 cases was significantly lower than that before treatment (P <0.01). Miscarriage success rate of 92.59%. Conclusion: The proportion of CD4 + CD25 + FOXP3 + Treg cells in peripheral blood of patients with ACA-positive threatened abortion is lower than that of normal pregnant women, and there is abnormal immunoregulation. One of the mechanisms of treatment of ACA-positive threatened abortion by " Treg cell ratio, regulating immune function in pregnancy related.