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采集新疆喀什、阿克苏、鄯善、吐鲁番、哈密、昌吉、博乐、伊犁的棉铃虫幼虫,人工饲养建立8个棉铃虫地理种群,通过测定棉铃虫不同地理种群滞育蛹的过冷却点、低温生存能力(LT_(50))及血淋巴中糖含量来比较不同地理种群棉铃虫的耐寒性。利用Logger-220过冷却仪对棉铃虫过冷却点的测定结果表明,新疆不同地理种群棉铃虫之间过冷却点差异不显著(P>0.05)。新疆不同地理种群棉铃虫的滞育蛹在-15℃和-20℃的室内条件下,50%个体致死所需的时间(LT_(50))分别为13.0d和0.4d,种群间差异不显著(P>0.05)。新疆不同地理种群棉铃虫滞育蛹血淋巴中的含糖量测定结果表明,不同种群间差异不显著(P>0.05)。新疆棉铃虫喀什、阿克苏、鄯善、吐鲁番、哈密、昌吉、博乐、伊犁种群雌虫滞育蛹血淋巴含糖量为2.72~3.10 mg/100mg,雄虫为2.65~3.02mg/100mg。新疆不同地理种群棉铃虫的耐寒性不存在差别。
The cotton bollworm larvae were collected from Kashgar, Aksu, Shanshan, Turpan, Hami, Changji, Bole and Ili in Xinjiang. Eight geographical populations of H. armigera were established by artificial feeding. The subcooling point of diapause pupae in different geographical populations of cotton bollworm was determined. Viability (LT_ (50)) and hemolymph sugar content were compared to compare the cold tolerance of different geographical populations of cotton bollworm. The results of supercooled point of cotton bollworm using Logger-220 supercooler showed that there was no significant difference (P> 0.05) between the subcooling points of cotton bollworm in different geographical populations in Xinjiang. Diapause pupae of cotton bollworm (Helicoverpa armigera larvae) from different geographical populations in Xinjiang at indoor temperature of -15 ℃ and -20 ℃ showed that the time required for lethality of 50% of individuals (LT 50) was 13.0d and 0.4d respectively, with no significant difference among the populations (P> 0.05). The results showed that there was no significant difference among the different populations (P> 0.05). The hemolymph of diapause pupae of cotton bollworm in Kashgar, Aksu, Shanshan, Turpan, Hami, Changji, Bole and Yili of Xinjiang was 2.72-3.10 mg / 100 mg and the male was 2.65-3.02 mg / 100 mg. Cold tolerance of cotton bollworm in different geographical populations in Xinjiang is not different.