Objective To investigate the level of antisperm antibodies(ASA) in infertile men with absolute immotile sperm(AIS).Methods Thirteen men with AIS were enrolled into this study. Sperm immotility of ejaculates from these infertile men was proven by two or three consecutive semen analyses. Sperm vitality was assessed by the eosin exclusion. Serum and seminal plasma were screened by the indirect immunobead test(IBT) for ASA type IgG and IgA according to the WHO laboratory manual.Results Semen samples from these patients showed ejaculate volume, pH, and sperm count in the normal range. Sperm vitality, sperm concentration, and sperm abnormal forms in these cases were 40.2 ± 11.1%, 45.0 ± 11.9 × 106/ml, and 97.8 ± 1.4%,respectively. No case had the positive or sub-positive level of ASA type IgG and IgA in samples of both serum and seminal plasma.Conclusion ASA is not associated with the pathogenesis of AIS-associated infertility. Objective To investigate the level of antisperm antibodies (ASA) in infertile men with absolute immotile sperm (AIS). Methods Thirteen men with AIS were enrolled into this study. Sperm immotility of ejaculates from these infertile men was proven by two or three consecutive semen analyzes Serum and seminal plasma were screened by the indirect immunobead test (IBT) for ASA type IgG and IgA according to the WHO laboratory manual. Results Semen samples from these patients showed ejaculate volume, pH, and Sperm count in the normal range. Sperm concentration, and sperm abnormal forms in these cases were 40.2 ± 11.1%, 45.0 ± 11.9 × 106 / ml, and 97.8 ± 1.4%, respectively. No case had the positive or sub- positive level of ASA type IgG and IgA in samples of both serum and seminal plasma. Confluence ASA is not associated with the pathogenesis of AIS-associated infertility.