论文部分内容阅读
为了拓展微结构聚合物光纤(MPOF)在化学传感领域内的应用研究以及对MPOF孔道内壁功能化修饰技术的深入研究,首先对36孔的MPOF孔道内壁进行酸催化水解处理,随后采用化学键合的方式将荧光探针曙红通过偶联剂负载于MPOF孔道内壁,从而制成用于pH值测定的传感探头。该传感探头被修饰后的多个孔道可以容纳微量的待测溶液,待测物与孔道内壁的荧光探针作用后,光信号将可以沿光纤传输,并被检测。整个化学反应的传感过程是在孔道内进行的,传导的光束能够和敏感材料相重叠,利于微量分析同时避免了对敏感膜的损坏。实验结果显示pH值在2.0～4.4范围内与荧光强度呈线性关系,该传感探头的重现性良好,并且采用此修饰方法制备的MPOF传感探头对于荧光探针的固定性比较好,无明显泄漏。
In order to explore the application of MPOF in the field of chemical sensing and further study on the functional modification of MPOF pore, the inner wall of MPOF pore in 36-well was acid-catalyzed and hydrolyzed first, followed by chemical bonding The fluorescent probe eosin was loaded on the inner wall of the MPOF pore through a coupling agent to prepare a sensing probe for pH measurement. After being modified, a plurality of channels can accommodate a small amount of solution to be measured. After the analyte interacts with the fluorescent probe on the inner wall of the channel, the optical signal can be transmitted along the optical fiber and detected. The entire chemical reaction sensing process is carried out in the tunnel, the conductive light beam and sensitive material overlap, which is conducive to trace analysis and avoid the damage to the sensitive membrane. The experimental results show that the pH value is linear with the fluorescence intensity in the range of 2.0 to 4.4, the reproducibility of the sensor is good, and the MPOF sensor probe prepared by using the modified method has better fixability to the fluorescent probe, and no Clearly leaked.